Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/leftover/EPI-44_S41_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/leftover/EPI-44_S41_L005_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 13762658 Number of paired-end alignments with a unique best hit: 7733705 Mapping efficiency: 56.2% Sequence pairs with no alignments under any condition: 3651938 Sequence pairs did not map uniquely: 2377015 Sequence pairs which were discarded because genomic sequence could not be extracted: 31800 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3832028 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 3869877 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 228132333 Total methylated C's in CpG context: 9273623 Total methylated C's in CHG context: 1279711 Total methylated C's in CHH context: 7254809 Total methylated C's in Unknown context: 225878 Total unmethylated C's in CpG context: 22503284 Total unmethylated C's in CHG context: 47720775 Total unmethylated C's in CHH context: 140100131 Total unmethylated C's in Unknown context: 965417 C methylated in CpG context: 29.2% C methylated in CHG context: 2.6% C methylated in CHH context: 4.9% C methylated in unknown context (CN or CHN): 19.0% Bismark completed in 0d 2h 53m 14s