Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-206_S27_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-206_S27_L004_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 20931018 Number of paired-end alignments with a unique best hit: 11521920 Mapping efficiency: 55.0% Sequence pairs with no alignments under any condition: 5600370 Sequence pairs did not map uniquely: 3808728 Sequence pairs which were discarded because genomic sequence could not be extracted: 45946 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5661049 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 5814925 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 353645201 Total methylated C's in CpG context: 11668408 Total methylated C's in CHG context: 1871252 Total methylated C's in CHH context: 7299277 Total methylated C's in Unknown context: 237164 Total unmethylated C's in CpG context: 44163722 Total unmethylated C's in CHG context: 77079068 Total unmethylated C's in CHH context: 211563474 Total unmethylated C's in Unknown context: 1710102 C methylated in CpG context: 20.9% C methylated in CHG context: 2.4% C methylated in CHH context: 3.3% C methylated in unknown context (CN or CHN): 12.2% Bismark completed in 0d 4h 16m 30s