Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-199_S24_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-199_S24_L003_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 15913069 Number of paired-end alignments with a unique best hit: 8550693 Mapping efficiency: 53.7% Sequence pairs with no alignments under any condition: 4729683 Sequence pairs did not map uniquely: 2632693 Sequence pairs which were discarded because genomic sequence could not be extracted: 45046 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4208172 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 4297475 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 259990101 Total methylated C's in CpG context: 9492646 Total methylated C's in CHG context: 1543069 Total methylated C's in CHH context: 9796003 Total methylated C's in Unknown context: 329556 Total unmethylated C's in CpG context: 28236558 Total unmethylated C's in CHG context: 55100132 Total unmethylated C's in CHH context: 155821693 Total unmethylated C's in Unknown context: 1311028 C methylated in CpG context: 25.2% C methylated in CHG context: 2.7% C methylated in CHH context: 5.9% C methylated in unknown context (CN or CHN): 20.1% Bismark completed in 0d 3h 13m 3s