Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-188_S21_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-188_S21_L003_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 21488138 Number of paired-end alignments with a unique best hit: 10978019 Mapping efficiency: 51.1% Sequence pairs with no alignments under any condition: 6944690 Sequence pairs did not map uniquely: 3565429 Sequence pairs which were discarded because genomic sequence could not be extracted: 56248 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5422254 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 5499517 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 330933327 Total methylated C's in CpG context: 10013389 Total methylated C's in CHG context: 1746730 Total methylated C's in CHH context: 6803412 Total methylated C's in Unknown context: 219928 Total unmethylated C's in CpG context: 40180946 Total unmethylated C's in CHG context: 70497987 Total unmethylated C's in CHH context: 201690863 Total unmethylated C's in Unknown context: 1659459 C methylated in CpG context: 19.9% C methylated in CHG context: 2.4% C methylated in CHH context: 3.3% C methylated in unknown context (CN or CHN): 11.7% Bismark completed in 0d 4h 3m 3s