Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-187_S20_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-187_S20_L003_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 22734395 Number of paired-end alignments with a unique best hit: 13573422 Mapping efficiency: 59.7% Sequence pairs with no alignments under any condition: 5092561 Sequence pairs did not map uniquely: 4068412 Sequence pairs which were discarded because genomic sequence could not be extracted: 62145 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 6664215 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 6847062 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 425504629 Total methylated C's in CpG context: 15084090 Total methylated C's in CHG context: 2281665 Total methylated C's in CHH context: 8712838 Total methylated C's in Unknown context: 265203 Total unmethylated C's in CpG context: 48860840 Total unmethylated C's in CHG context: 92161675 Total unmethylated C's in CHH context: 258403521 Total unmethylated C's in Unknown context: 1997501 C methylated in CpG context: 23.6% C methylated in CHG context: 2.4% C methylated in CHH context: 3.3% C methylated in unknown context (CN or CHN): 11.7% Bismark completed in 0d 5h 8m 44s