Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-159_S6_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-159_S6_L002_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 15453987 Number of paired-end alignments with a unique best hit: 9136462 Mapping efficiency: 59.1% Sequence pairs with no alignments under any condition: 3665126 Sequence pairs did not map uniquely: 2652399 Sequence pairs which were discarded because genomic sequence could not be extracted: 55159 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4472321 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 4608982 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 289174054 Total methylated C's in CpG context: 9492162 Total methylated C's in CHG context: 1624273 Total methylated C's in CHH context: 9702386 Total methylated C's in Unknown context: 300927 Total unmethylated C's in CpG context: 31891715 Total unmethylated C's in CHG context: 60744506 Total unmethylated C's in CHH context: 175719012 Total unmethylated C's in Unknown context: 1466391 C methylated in CpG context: 22.9% C methylated in CHG context: 2.6% C methylated in CHH context: 5.2% C methylated in unknown context (CN or CHN): 17.0% Bismark completed in 0d 3h 27m 22s