Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-152_S3_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-152_S3_L002_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 29855018 Number of paired-end alignments with a unique best hit: 17480743 Mapping efficiency: 58.6% Sequence pairs with no alignments under any condition: 7291555 Sequence pairs did not map uniquely: 5082720 Sequence pairs which were discarded because genomic sequence could not be extracted: 91230 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7949395 ((converted) top strand) GA/CT/CT: 633023 (complementary to (converted) top strand) GA/CT/GA: 649555 (complementary to (converted) bottom strand) CT/GA/GA: 8157540 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 551826884 Total methylated C's in CpG context: 18057953 Total methylated C's in CHG context: 2990393 Total methylated C's in CHH context: 27434841 Total methylated C's in Unknown context: 1000667 Total unmethylated C's in CpG context: 57333026 Total unmethylated C's in CHG context: 111361117 Total unmethylated C's in CHH context: 334649554 Total unmethylated C's in Unknown context: 3107316 C methylated in CpG context: 24.0% C methylated in CHG context: 2.6% C methylated in CHH context: 7.6% C methylated in unknown context (CN or CHN): 24.4% Bismark completed in 0d 8h 47m 54s