Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-135_S35_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-135_S35_L005_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 28731808 Number of paired-end alignments with a unique best hit: 18358571 Mapping efficiency: 63.9% Sequence pairs with no alignments under any condition: 4893497 Sequence pairs did not map uniquely: 5479740 Sequence pairs which were discarded because genomic sequence could not be extracted: 67150 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8411112 ((converted) top strand) GA/CT/CT: 631208 (complementary to (converted) top strand) GA/CT/GA: 650614 (complementary to (converted) bottom strand) CT/GA/GA: 8598487 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 570220880 Total methylated C's in CpG context: 17195669 Total methylated C's in CHG context: 3225591 Total methylated C's in CHH context: 23525793 Total methylated C's in Unknown context: 858420 Total unmethylated C's in CpG context: 63237677 Total unmethylated C's in CHG context: 113217843 Total unmethylated C's in CHH context: 349818307 Total unmethylated C's in Unknown context: 2993331 C methylated in CpG context: 21.4% C methylated in CHG context: 2.8% C methylated in CHH context: 6.3% C methylated in unknown context (CN or CHN): 22.3% Bismark completed in 0d 8h 52m 46s