Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-120_S32_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-120_S32_L005_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 24140505 Number of paired-end alignments with a unique best hit: 15887911 Mapping efficiency: 65.8% Sequence pairs with no alignments under any condition: 3955980 Sequence pairs did not map uniquely: 4296614 Sequence pairs which were discarded because genomic sequence could not be extracted: 54868 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7495995 ((converted) top strand) GA/CT/CT: 369379 (complementary to (converted) top strand) GA/CT/GA: 376460 (complementary to (converted) bottom strand) CT/GA/GA: 7591209 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 506804444 Total methylated C's in CpG context: 14796422 Total methylated C's in CHG context: 2552556 Total methylated C's in CHH context: 17168742 Total methylated C's in Unknown context: 579759 Total unmethylated C's in CpG context: 54673696 Total unmethylated C's in CHG context: 101768912 Total unmethylated C's in CHH context: 315844116 Total unmethylated C's in Unknown context: 2606687 C methylated in CpG context: 21.3% C methylated in CHG context: 2.4% C methylated in CHH context: 5.2% C methylated in unknown context (CN or CHN): 18.2% Bismark completed in 0d 7h 48m 42s