Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-119_S31_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-119_S31_L005_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 28905983 Number of paired-end alignments with a unique best hit: 18510081 Mapping efficiency: 64.0% Sequence pairs with no alignments under any condition: 5236342 Sequence pairs did not map uniquely: 5159560 Sequence pairs which were discarded because genomic sequence could not be extracted: 55302 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8628401 ((converted) top strand) GA/CT/CT: 445598 (complementary to (converted) top strand) GA/CT/GA: 455153 (complementary to (converted) bottom strand) CT/GA/GA: 8925627 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 608728091 Total methylated C's in CpG context: 15666954 Total methylated C's in CHG context: 2916966 Total methylated C's in CHH context: 19314771 Total methylated C's in Unknown context: 692853 Total unmethylated C's in CpG context: 79114881 Total unmethylated C's in CHG context: 122174524 Total unmethylated C's in CHH context: 369539995 Total unmethylated C's in Unknown context: 3002445 C methylated in CpG context: 16.5% C methylated in CHG context: 2.3% C methylated in CHH context: 5.0% C methylated in unknown context (CN or CHN): 18.7% Bismark completed in 0d 8h 38m 19s