Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-113_S30_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-113_S30_L005_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 26302191 Number of paired-end alignments with a unique best hit: 17092677 Mapping efficiency: 65.0% Sequence pairs with no alignments under any condition: 4410947 Sequence pairs did not map uniquely: 4798567 Sequence pairs which were discarded because genomic sequence could not be extracted: 59343 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7976122 ((converted) top strand) GA/CT/CT: 424828 (complementary to (converted) top strand) GA/CT/GA: 437389 (complementary to (converted) bottom strand) CT/GA/GA: 8194995 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 549576239 Total methylated C's in CpG context: 15464165 Total methylated C's in CHG context: 2791089 Total methylated C's in CHH context: 19091793 Total methylated C's in Unknown context: 663981 Total unmethylated C's in CpG context: 62810651 Total unmethylated C's in CHG context: 110115384 Total unmethylated C's in CHH context: 339303157 Total unmethylated C's in Unknown context: 2800953 C methylated in CpG context: 19.8% C methylated in CHG context: 2.5% C methylated in CHH context: 5.3% C methylated in unknown context (CN or CHN): 19.2% Bismark completed in 0d 7h 47m 58s