Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-111_S29_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-111_S29_L005_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 27014996 Number of paired-end alignments with a unique best hit: 17470998 Mapping efficiency: 64.7% Sequence pairs with no alignments under any condition: 4681660 Sequence pairs did not map uniquely: 4862338 Sequence pairs which were discarded because genomic sequence could not be extracted: 66653 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8171153 ((converted) top strand) GA/CT/CT: 475619 (complementary to (converted) top strand) GA/CT/GA: 485354 (complementary to (converted) bottom strand) CT/GA/GA: 8272219 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 549392843 Total methylated C's in CpG context: 16051052 Total methylated C's in CHG context: 2847593 Total methylated C's in CHH context: 19507880 Total methylated C's in Unknown context: 725557 Total unmethylated C's in CpG context: 58496439 Total unmethylated C's in CHG context: 109735777 Total unmethylated C's in CHH context: 342754102 Total unmethylated C's in Unknown context: 2776371 C methylated in CpG context: 21.5% C methylated in CHG context: 2.5% C methylated in CHH context: 5.4% C methylated in unknown context (CN or CHN): 20.7% Bismark completed in 0d 8h 32m 19s