Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-103_S27_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-103_S27_L005_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 20349659 Number of paired-end alignments with a unique best hit: 13364383 Mapping efficiency: 65.7% Sequence pairs with no alignments under any condition: 3385894 Sequence pairs did not map uniquely: 3599382 Sequence pairs which were discarded because genomic sequence could not be extracted: 45055 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 6344954 ((converted) top strand) GA/CT/CT: 288878 (complementary to (converted) top strand) GA/CT/GA: 294928 (complementary to (converted) bottom strand) CT/GA/GA: 6390568 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 436619499 Total methylated C's in CpG context: 12108570 Total methylated C's in CHG context: 2075398 Total methylated C's in CHH context: 14520988 Total methylated C's in Unknown context: 485587 Total unmethylated C's in CpG context: 48927920 Total unmethylated C's in CHG context: 89229392 Total unmethylated C's in CHH context: 269757231 Total unmethylated C's in Unknown context: 2195501 C methylated in CpG context: 19.8% C methylated in CHG context: 2.3% C methylated in CHH context: 5.1% C methylated in unknown context (CN or CHN): 18.1% Bismark completed in 0d 6h 36m 43s