Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-227_S35_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-227_S35_L004_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 6313 Mapping efficiency: 63.1% Sequence pairs with no alignments under any condition: 1859 Sequence pairs did not map uniquely: 1828 Sequence pairs which were discarded because genomic sequence could not be extracted: 22 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2801 ((converted) top strand) GA/CT/CT: 343 (complementary to (converted) top strand) GA/CT/GA: 327 (complementary to (converted) bottom strand) CT/GA/GA: 2820 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 194847 Total methylated C's in CpG context: 6521 Total methylated C's in CHG context: 1098 Total methylated C's in CHH context: 11910 Total methylated C's in Unknown context: 430 Total unmethylated C's in CpG context: 20032 Total unmethylated C's in CHG context: 38832 Total unmethylated C's in CHH context: 116454 Total unmethylated C's in Unknown context: 1106 C methylated in CpG context: 24.6% C methylated in CHG context: 2.7% C methylated in CHH context: 9.3% C methylated in unknown context (CN or CHN): 28.0% Bismark completed in 0d 0h 0m 58s