Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-205_S26_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-205_S26_L004_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 5560 Mapping efficiency: 55.6% Sequence pairs with no alignments under any condition: 2442 Sequence pairs did not map uniquely: 1998 Sequence pairs which were discarded because genomic sequence could not be extracted: 26 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2342 ((converted) top strand) GA/CT/CT: 435 (complementary to (converted) top strand) GA/CT/GA: 432 (complementary to (converted) bottom strand) CT/GA/GA: 2325 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 161906 Total methylated C's in CpG context: 5167 Total methylated C's in CHG context: 1131 Total methylated C's in CHH context: 12325 Total methylated C's in Unknown context: 542 Total unmethylated C's in CpG context: 17866 Total unmethylated C's in CHG context: 31647 Total unmethylated C's in CHH context: 93770 Total unmethylated C's in Unknown context: 1098 C methylated in CpG context: 22.4% C methylated in CHG context: 3.5% C methylated in CHH context: 11.6% C methylated in unknown context (CN or CHN): 33.0% Bismark completed in 0d 0h 0m 52s