Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-188_S21_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-188_S21_L003_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 5670 Mapping efficiency: 56.7% Sequence pairs with no alignments under any condition: 2521 Sequence pairs did not map uniquely: 1809 Sequence pairs which were discarded because genomic sequence could not be extracted: 22 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2443 ((converted) top strand) GA/CT/CT: 345 (complementary to (converted) top strand) GA/CT/GA: 364 (complementary to (converted) bottom strand) CT/GA/GA: 2496 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 167150 Total methylated C's in CpG context: 4841 Total methylated C's in CHG context: 992 Total methylated C's in CHH context: 9590 Total methylated C's in Unknown context: 461 Total unmethylated C's in CpG context: 19033 Total unmethylated C's in CHG context: 33458 Total unmethylated C's in CHH context: 99236 Total unmethylated C's in Unknown context: 1032 C methylated in CpG context: 20.3% C methylated in CHG context: 2.9% C methylated in CHH context: 8.8% C methylated in unknown context (CN or CHN): 30.9% Bismark completed in 0d 0h 0m 53s