Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-167_S10_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-167_S10_L002_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 6346 Mapping efficiency: 63.5% Sequence pairs with no alignments under any condition: 1856 Sequence pairs did not map uniquely: 1798 Sequence pairs which were discarded because genomic sequence could not be extracted: 39 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2950 ((converted) top strand) GA/CT/CT: 206 (complementary to (converted) top strand) GA/CT/GA: 201 (complementary to (converted) bottom strand) CT/GA/GA: 2950 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 204370 Total methylated C's in CpG context: 6423 Total methylated C's in CHG context: 1384 Total methylated C's in CHH context: 9741 Total methylated C's in Unknown context: 280 Total unmethylated C's in CpG context: 21477 Total unmethylated C's in CHG context: 42067 Total unmethylated C's in CHH context: 123278 Total unmethylated C's in Unknown context: 1147 C methylated in CpG context: 23.0% C methylated in CHG context: 3.2% C methylated in CHH context: 7.3% C methylated in unknown context (CN or CHN): 19.6% Bismark completed in 0d 0h 1m 2s