Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-119_S31_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-119_S31_L005_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 6398 Mapping efficiency: 64.0% Sequence pairs with no alignments under any condition: 1792 Sequence pairs did not map uniquely: 1810 Sequence pairs which were discarded because genomic sequence could not be extracted: 17 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2937 ((converted) top strand) GA/CT/CT: 152 (complementary to (converted) top strand) GA/CT/GA: 158 (complementary to (converted) bottom strand) CT/GA/GA: 3134 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 211063 Total methylated C's in CpG context: 5513 Total methylated C's in CHG context: 1027 Total methylated C's in CHH context: 6114 Total methylated C's in Unknown context: 209 Total unmethylated C's in CpG context: 27762 Total unmethylated C's in CHG context: 42644 Total unmethylated C's in CHH context: 128003 Total unmethylated C's in Unknown context: 1057 C methylated in CpG context: 16.6% C methylated in CHG context: 2.4% C methylated in CHH context: 4.6% C methylated in unknown context (CN or CHN): 16.5% Bismark completed in 0d 0h 0m 54s