Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-104_S28_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-104_S28_L005_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 6548 Mapping efficiency: 65.5% Sequence pairs with no alignments under any condition: 1621 Sequence pairs did not map uniquely: 1831 Sequence pairs which were discarded because genomic sequence could not be extracted: 14 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3080 ((converted) top strand) GA/CT/CT: 161 (complementary to (converted) top strand) GA/CT/GA: 202 (complementary to (converted) bottom strand) CT/GA/GA: 3091 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 205827 Total methylated C's in CpG context: 6353 Total methylated C's in CHG context: 952 Total methylated C's in CHH context: 7875 Total methylated C's in Unknown context: 257 Total unmethylated C's in CpG context: 21220 Total unmethylated C's in CHG context: 41078 Total unmethylated C's in CHH context: 128349 Total unmethylated C's in Unknown context: 1030 C methylated in CpG context: 23.0% C methylated in CHG context: 2.3% C methylated in CHH context: 5.8% C methylated in unknown context (CN or CHN): 20.0% Bismark completed in 0d 0h 0m 53s