Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-44_S41_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-44_S41_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6160
Pairs without alignments under any condition 1937
Pairs that did not map uniquely 1903
Genomic sequence context not extractable (edges of chromosomes) 24

Cytosine Methylation

Total C's analysed 178943
Methylated C's in CpG context 6808
Methylated C's in CHG context 1030
Methylated C's in CHH context 10522
Methylated C's in Unknown context 431
Unmethylated C's in CpG context 16703
Unmethylated C's in CHG context 34940
Unmethylated C's in CHH context 108940
Unmethylated C's in Unknown context 981
Percentage methylation (CpG context) 29.0%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 8.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2655 original top strand
CTOT 381 complementary to original top strand
CTOB 362 complementary to original bottom strand
OB 2738 original bottom strand

Deduplication

Alignments analysed 6136
Unique alignments 6119
Duplicates removed 17
Duplicated alignments were found at 14 different positions

Cytosine Methylation after Extraction

Total C's analysed 130797
Methylated C's in CpG context 4929
Methylated C's in CHG context 796
Methylated C's in CHH context 8252
Unmethylated C's in CpG context 12034
Unmethylated C's in CHG context 25286
Unmethylated C's in CHH context 79500
Percentage methylation (CpG context) 29.1%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 9.4%

M-Bias Plot