Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-43_S40_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-43_S40_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6210
Pairs without alignments under any condition 1863
Pairs that did not map uniquely 1927
Genomic sequence context not extractable (edges of chromosomes) 22

Cytosine Methylation

Total C's analysed 186153
Methylated C's in CpG context 6487
Methylated C's in CHG context 1015
Methylated C's in CHH context 11248
Methylated C's in Unknown context 369
Unmethylated C's in CpG context 18210
Unmethylated C's in CHG context 36502
Unmethylated C's in CHH context 112691
Unmethylated C's in Unknown context 1069
Percentage methylation (CpG context) 26.3%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 9.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2805 original top strand
CTOT 315 complementary to original top strand
CTOB 344 complementary to original bottom strand
OB 2724 original bottom strand

Deduplication

Alignments analysed 6188
Unique alignments 6173
Duplicates removed 15
Duplicated alignments were found at 10 different positions

Cytosine Methylation after Extraction

Total C's analysed 135440
Methylated C's in CpG context 4721
Methylated C's in CHG context 794
Methylated C's in CHH context 8288
Unmethylated C's in CpG context 13281
Unmethylated C's in CHG context 26359
Unmethylated C's in CHH context 81997
Percentage methylation (CpG context) 26.2%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 9.2%

M-Bias Plot