Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-42_S39_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-42_S39_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6178
Pairs without alignments under any condition 1925
Pairs that did not map uniquely 1897
Genomic sequence context not extractable (edges of chromosomes) 29

Cytosine Methylation

Total C's analysed 181548
Methylated C's in CpG context 6365
Methylated C's in CHG context 1121
Methylated C's in CHH context 11440
Methylated C's in Unknown context 442
Unmethylated C's in CpG context 17527
Unmethylated C's in CHG context 36119
Unmethylated C's in CHH context 108976
Unmethylated C's in Unknown context 1009
Percentage methylation (CpG context) 26.6%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 9.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2758 original top strand
CTOT 350 complementary to original top strand
CTOB 316 complementary to original bottom strand
OB 2725 original bottom strand

Deduplication

Alignments analysed 6149
Unique alignments 6127
Duplicates removed 22
Duplicated alignments were found at 15 different positions

Cytosine Methylation after Extraction

Total C's analysed 131176
Methylated C's in CpG context 4643
Methylated C's in CHG context 875
Methylated C's in CHH context 8664
Unmethylated C's in CpG context 12604
Unmethylated C's in CHG context 25715
Unmethylated C's in CHH context 78675
Percentage methylation (CpG context) 26.9%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 9.9%

M-Bias Plot