Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-41_S38_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-41_S38_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5916
Pairs without alignments under any condition 2213
Pairs that did not map uniquely 1871
Genomic sequence context not extractable (edges of chromosomes) 34

Cytosine Methylation

Total C's analysed 177246
Methylated C's in CpG context 6000
Methylated C's in CHG context 1112
Methylated C's in CHH context 12335
Methylated C's in Unknown context 466
Unmethylated C's in CpG context 17729
Unmethylated C's in CHG context 35239
Unmethylated C's in CHH context 104831
Unmethylated C's in Unknown context 1085
Percentage methylation (CpG context) 25.3%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 10.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2554 original top strand
CTOT 398 complementary to original top strand
CTOB 405 complementary to original bottom strand
OB 2525 original bottom strand

Deduplication

Alignments analysed 5882
Unique alignments 5851
Duplicates removed 31
Duplicated alignments were found at 21 different positions

Cytosine Methylation after Extraction

Total C's analysed 128811
Methylated C's in CpG context 4366
Methylated C's in CHG context 851
Methylated C's in CHH context 9460
Unmethylated C's in CpG context 12746
Unmethylated C's in CHG context 25232
Unmethylated C's in CHH context 76156
Percentage methylation (CpG context) 25.5%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 11.0%

M-Bias Plot