Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-230_S37_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-230_S37_L004_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5506
Pairs without alignments under any condition 2727
Pairs that did not map uniquely 1767
Genomic sequence context not extractable (edges of chromosomes) 23

Cytosine Methylation

Total C's analysed 165045
Methylated C's in CpG context 4957
Methylated C's in CHG context 893
Methylated C's in CHH context 9444
Methylated C's in Unknown context 408
Unmethylated C's in CpG context 18617
Unmethylated C's in CHG context 32962
Unmethylated C's in CHH context 98172
Unmethylated C's in Unknown context 1023
Percentage methylation (CpG context) 21.0%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 8.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2430 original top strand
CTOT 293 complementary to original top strand
CTOB 321 complementary to original bottom strand
OB 2439 original bottom strand

Deduplication

Alignments analysed 5483
Unique alignments 5437
Duplicates removed 46
Duplicated alignments were found at 28 different positions

Cytosine Methylation after Extraction

Total C's analysed 116536
Methylated C's in CpG context 3524
Methylated C's in CHG context 669
Methylated C's in CHH context 7116
Unmethylated C's in CpG context 12987
Unmethylated C's in CHG context 22778
Unmethylated C's in CHH context 69462
Percentage methylation (CpG context) 21.3%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 9.3%

M-Bias Plot