Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-229_S36_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-229_S36_L004_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6124
Pairs without alignments under any condition 1989
Pairs that did not map uniquely 1887
Genomic sequence context not extractable (edges of chromosomes) 23

Cytosine Methylation

Total C's analysed 189281
Methylated C's in CpG context 6217
Methylated C's in CHG context 1071
Methylated C's in CHH context 9366
Methylated C's in Unknown context 411
Unmethylated C's in CpG context 22113
Unmethylated C's in CHG context 38965
Unmethylated C's in CHH context 111549
Unmethylated C's in Unknown context 1220
Percentage methylation (CpG context) 21.9%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 7.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2666 original top strand
CTOT 311 complementary to original top strand
CTOB 332 complementary to original bottom strand
OB 2792 original bottom strand

Deduplication

Alignments analysed 6101
Unique alignments 6061
Duplicates removed 40
Duplicated alignments were found at 26 different positions

Cytosine Methylation after Extraction

Total C's analysed 135719
Methylated C's in CpG context 4464
Methylated C's in CHG context 810
Methylated C's in CHH context 7099
Unmethylated C's in CpG context 15758
Unmethylated C's in CHG context 27454
Unmethylated C's in CHH context 80134
Percentage methylation (CpG context) 22.1%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 8.1%

M-Bias Plot