Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-227_S35_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-227_S35_L004_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6313
Pairs without alignments under any condition 1859
Pairs that did not map uniquely 1828
Genomic sequence context not extractable (edges of chromosomes) 22

Cytosine Methylation

Total C's analysed 194847
Methylated C's in CpG context 6521
Methylated C's in CHG context 1098
Methylated C's in CHH context 11910
Methylated C's in Unknown context 430
Unmethylated C's in CpG context 20032
Unmethylated C's in CHG context 38832
Unmethylated C's in CHH context 116454
Unmethylated C's in Unknown context 1106
Percentage methylation (CpG context) 24.6%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 9.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2801 original top strand
CTOT 343 complementary to original top strand
CTOB 327 complementary to original bottom strand
OB 2820 original bottom strand

Deduplication

Alignments analysed 6291
Unique alignments 6253
Duplicates removed 38
Duplicated alignments were found at 24 different positions

Cytosine Methylation after Extraction

Total C's analysed 141005
Methylated C's in CpG context 4747
Methylated C's in CHG context 824
Methylated C's in CHH context 9029
Unmethylated C's in CpG context 14402
Unmethylated C's in CHG context 27698
Unmethylated C's in CHH context 84305
Percentage methylation (CpG context) 24.8%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 9.7%

M-Bias Plot