Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-221_S33_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-221_S33_L004_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6052
Pairs without alignments under any condition 1911
Pairs that did not map uniquely 2037
Genomic sequence context not extractable (edges of chromosomes) 29

Cytosine Methylation

Total C's analysed 180435
Methylated C's in CpG context 5937
Methylated C's in CHG context 1153
Methylated C's in CHH context 11247
Methylated C's in Unknown context 402
Unmethylated C's in CpG context 19015
Unmethylated C's in CHG context 35893
Unmethylated C's in CHH context 107190
Unmethylated C's in Unknown context 1162
Percentage methylation (CpG context) 23.8%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 9.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2569 original top strand
CTOT 368 complementary to original top strand
CTOB 386 complementary to original bottom strand
OB 2700 original bottom strand

Deduplication

Alignments analysed 6023
Unique alignments 5985
Duplicates removed 38
Duplicated alignments were found at 26 different positions

Cytosine Methylation after Extraction

Total C's analysed 130473
Methylated C's in CpG context 4314
Methylated C's in CHG context 884
Methylated C's in CHH context 8541
Unmethylated C's in CpG context 13600
Unmethylated C's in CHG context 25431
Unmethylated C's in CHH context 77703
Percentage methylation (CpG context) 24.1%
Percentage methylation (CHG context) 3.4%
Percentage methylation (CHH context) 9.9%

M-Bias Plot