Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-220_S32_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-220_S32_L004_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6209
Pairs without alignments under any condition 1855
Pairs that did not map uniquely 1936
Genomic sequence context not extractable (edges of chromosomes) 29

Cytosine Methylation

Total C's analysed 193726
Methylated C's in CpG context 5979
Methylated C's in CHG context 1141
Methylated C's in CHH context 10272
Methylated C's in Unknown context 385
Unmethylated C's in CpG context 22164
Unmethylated C's in CHG context 39425
Unmethylated C's in CHH context 114745
Unmethylated C's in Unknown context 1092
Percentage methylation (CpG context) 21.2%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 8.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2673 original top strand
CTOT 319 complementary to original top strand
CTOB 357 complementary to original bottom strand
OB 2831 original bottom strand

Deduplication

Alignments analysed 6180
Unique alignments 6114
Duplicates removed 66
Duplicated alignments were found at 40 different positions

Cytosine Methylation after Extraction

Total C's analysed 138187
Methylated C's in CpG context 4310
Methylated C's in CHG context 855
Methylated C's in CHH context 7952
Unmethylated C's in CpG context 15507
Unmethylated C's in CHG context 27620
Unmethylated C's in CHH context 81943
Percentage methylation (CpG context) 21.7%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 8.8%

M-Bias Plot