Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-215_S31_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-215_S31_L004_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6061
Pairs without alignments under any condition 2014
Pairs that did not map uniquely 1925
Genomic sequence context not extractable (edges of chromosomes) 34

Cytosine Methylation

Total C's analysed 182434
Methylated C's in CpG context 6338
Methylated C's in CHG context 1126
Methylated C's in CHH context 9585
Methylated C's in Unknown context 412
Unmethylated C's in CpG context 19112
Unmethylated C's in CHG context 37152
Unmethylated C's in CHH context 109121
Unmethylated C's in Unknown context 1087
Percentage methylation (CpG context) 24.9%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 8.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2666 original top strand
CTOT 318 complementary to original top strand
CTOB 331 complementary to original bottom strand
OB 2712 original bottom strand

Deduplication

Alignments analysed 6027
Unique alignments 5984
Duplicates removed 43
Duplicated alignments were found at 20 different positions

Cytosine Methylation after Extraction

Total C's analysed 131148
Methylated C's in CpG context 4621
Methylated C's in CHG context 872
Methylated C's in CHH context 7481
Unmethylated C's in CpG context 13500
Unmethylated C's in CHG context 26149
Unmethylated C's in CHH context 78525
Percentage methylation (CpG context) 25.5%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 8.7%

M-Bias Plot