Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-214_S30_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-214_S30_L004_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5860
Pairs without alignments under any condition 2362
Pairs that did not map uniquely 1778
Genomic sequence context not extractable (edges of chromosomes) 34

Cytosine Methylation

Total C's analysed 182994
Methylated C's in CpG context 5549
Methylated C's in CHG context 1002
Methylated C's in CHH context 9397
Methylated C's in Unknown context 359
Unmethylated C's in CpG context 19690
Unmethylated C's in CHG context 36943
Unmethylated C's in CHH context 110413
Unmethylated C's in Unknown context 948
Percentage methylation (CpG context) 22.0%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 7.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2551 original top strand
CTOT 275 complementary to original top strand
CTOB 249 complementary to original bottom strand
OB 2751 original bottom strand

Deduplication

Alignments analysed 5826
Unique alignments 5765
Duplicates removed 61
Duplicated alignments were found at 37 different positions

Cytosine Methylation after Extraction

Total C's analysed 131530
Methylated C's in CpG context 4057
Methylated C's in CHG context 764
Methylated C's in CHH context 6831
Unmethylated C's in CpG context 14103
Unmethylated C's in CHG context 26141
Unmethylated C's in CHH context 79634
Percentage methylation (CpG context) 22.3%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 7.9%

M-Bias Plot