Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-206_S27_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-206_S27_L004_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6085
Pairs without alignments under any condition 1898
Pairs that did not map uniquely 2017
Genomic sequence context not extractable (edges of chromosomes) 27

Cytosine Methylation

Total C's analysed 183834
Methylated C's in CpG context 5683
Methylated C's in CHG context 1054
Methylated C's in CHH context 10504
Methylated C's in Unknown context 370
Unmethylated C's in CpG context 22049
Unmethylated C's in CHG context 37149
Unmethylated C's in CHH context 107395
Unmethylated C's in Unknown context 1039
Percentage methylation (CpG context) 20.5%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 8.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2589 original top strand
CTOT 368 complementary to original top strand
CTOB 397 complementary to original bottom strand
OB 2704 original bottom strand

Deduplication

Alignments analysed 6058
Unique alignments 6025
Duplicates removed 33
Duplicated alignments were found at 27 different positions

Cytosine Methylation after Extraction

Total C's analysed 132010
Methylated C's in CpG context 4142
Methylated C's in CHG context 817
Methylated C's in CHH context 8090
Unmethylated C's in CpG context 15617
Unmethylated C's in CHG context 26192
Unmethylated C's in CHH context 77152
Percentage methylation (CpG context) 21.0%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 9.5%

M-Bias Plot