Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-200_S25_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-200_S25_L003_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5958
Pairs without alignments under any condition 2104
Pairs that did not map uniquely 1938
Genomic sequence context not extractable (edges of chromosomes) 20

Cytosine Methylation

Total C's analysed 174908
Methylated C's in CpG context 5732
Methylated C's in CHG context 1045
Methylated C's in CHH context 11140
Methylated C's in Unknown context 434
Unmethylated C's in CpG context 17224
Unmethylated C's in CHG context 35014
Unmethylated C's in CHH context 104753
Unmethylated C's in Unknown context 1118
Percentage methylation (CpG context) 25.0%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 9.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2648 original top strand
CTOT 358 complementary to original top strand
CTOB 352 complementary to original bottom strand
OB 2580 original bottom strand

Deduplication

Alignments analysed 5938
Unique alignments 5900
Duplicates removed 38
Duplicated alignments were found at 25 different positions

Cytosine Methylation after Extraction

Total C's analysed 125539
Methylated C's in CpG context 4113
Methylated C's in CHG context 805
Methylated C's in CHH context 8542
Unmethylated C's in CpG context 12207
Unmethylated C's in CHG context 24688
Unmethylated C's in CHH context 75184
Percentage methylation (CpG context) 25.2%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 10.2%

M-Bias Plot