Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-199_S24_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-199_S24_L003_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5842
Pairs without alignments under any condition 2259
Pairs that did not map uniquely 1899
Genomic sequence context not extractable (edges of chromosomes) 36

Cytosine Methylation

Total C's analysed 177634
Methylated C's in CpG context 6014
Methylated C's in CHG context 1082
Methylated C's in CHH context 11932
Methylated C's in Unknown context 428
Unmethylated C's in CpG context 18826
Unmethylated C's in CHG context 35913
Unmethylated C's in CHH context 103867
Unmethylated C's in Unknown context 1053
Percentage methylation (CpG context) 24.2%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 10.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2481 original top strand
CTOT 394 complementary to original top strand
CTOB 375 complementary to original bottom strand
OB 2556 original bottom strand

Deduplication

Alignments analysed 5806
Unique alignments 5781
Duplicates removed 25
Duplicated alignments were found at 17 different positions

Cytosine Methylation after Extraction

Total C's analysed 129089
Methylated C's in CpG context 4413
Methylated C's in CHG context 825
Methylated C's in CHH context 8954
Unmethylated C's in CpG context 13577
Unmethylated C's in CHG context 25634
Unmethylated C's in CHH context 75686
Percentage methylation (CpG context) 24.5%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 10.6%

M-Bias Plot