Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-194_S23_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-194_S23_L003_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6299
Pairs without alignments under any condition 1918
Pairs that did not map uniquely 1783
Genomic sequence context not extractable (edges of chromosomes) 28

Cytosine Methylation

Total C's analysed 202085
Methylated C's in CpG context 6978
Methylated C's in CHG context 998
Methylated C's in CHH context 10420
Methylated C's in Unknown context 309
Unmethylated C's in CpG context 21113
Unmethylated C's in CHG context 41596
Unmethylated C's in CHH context 120980
Unmethylated C's in Unknown context 1089
Percentage methylation (CpG context) 24.8%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 7.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2951 original top strand
CTOT 200 complementary to original top strand
CTOB 227 complementary to original bottom strand
OB 2893 original bottom strand

Deduplication

Alignments analysed 6271
Unique alignments 6237
Duplicates removed 34
Duplicated alignments were found at 29 different positions

Cytosine Methylation after Extraction

Total C's analysed 151678
Methylated C's in CpG context 5329
Methylated C's in CHG context 770
Methylated C's in CHH context 7625
Unmethylated C's in CpG context 15943
Unmethylated C's in CHG context 30790
Unmethylated C's in CHH context 91221
Percentage methylation (CpG context) 25.1%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 7.7%

M-Bias Plot