Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-193_S22_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-193_S22_L003_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5757
Pairs without alignments under any condition 2468
Pairs that did not map uniquely 1775
Genomic sequence context not extractable (edges of chromosomes) 36

Cytosine Methylation

Total C's analysed 171199
Methylated C's in CpG context 5037
Methylated C's in CHG context 983
Methylated C's in CHH context 10334
Methylated C's in Unknown context 393
Unmethylated C's in CpG context 17970
Unmethylated C's in CHG context 34380
Unmethylated C's in CHH context 102495
Unmethylated C's in Unknown context 1068
Percentage methylation (CpG context) 21.9%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 9.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2514 original top strand
CTOT 307 complementary to original top strand
CTOB 375 complementary to original bottom strand
OB 2525 original bottom strand

Deduplication

Alignments analysed 5721
Unique alignments 5670
Duplicates removed 51
Duplicated alignments were found at 31 different positions

Cytosine Methylation after Extraction

Total C's analysed 122182
Methylated C's in CpG context 3612
Methylated C's in CHG context 745
Methylated C's in CHH context 7957
Unmethylated C's in CpG context 12685
Unmethylated C's in CHG context 23942
Unmethylated C's in CHH context 73241
Percentage methylation (CpG context) 22.2%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 9.8%

M-Bias Plot