Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-188_S21_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-188_S21_L003_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5670
Pairs without alignments under any condition 2521
Pairs that did not map uniquely 1809
Genomic sequence context not extractable (edges of chromosomes) 22

Cytosine Methylation

Total C's analysed 167150
Methylated C's in CpG context 4841
Methylated C's in CHG context 992
Methylated C's in CHH context 9590
Methylated C's in Unknown context 461
Unmethylated C's in CpG context 19033
Unmethylated C's in CHG context 33458
Unmethylated C's in CHH context 99236
Unmethylated C's in Unknown context 1032
Percentage methylation (CpG context) 20.3%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 8.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2443 original top strand
CTOT 345 complementary to original top strand
CTOB 364 complementary to original bottom strand
OB 2496 original bottom strand

Deduplication

Alignments analysed 5648
Unique alignments 5595
Duplicates removed 53
Duplicated alignments were found at 29 different positions

Cytosine Methylation after Extraction

Total C's analysed 118315
Methylated C's in CpG context 3455
Methylated C's in CHG context 743
Methylated C's in CHH context 7730
Unmethylated C's in CpG context 13141
Unmethylated C's in CHG context 23068
Unmethylated C's in CHH context 70178
Percentage methylation (CpG context) 20.8%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 9.9%

M-Bias Plot