Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-187_S20_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-187_S20_L003_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6396
Pairs without alignments under any condition 1731
Pairs that did not map uniquely 1873
Genomic sequence context not extractable (edges of chromosomes) 29

Cytosine Methylation

Total C's analysed 201849
Methylated C's in CpG context 6691
Methylated C's in CHG context 1111
Methylated C's in CHH context 10221
Methylated C's in Unknown context 352
Unmethylated C's in CpG context 22198
Unmethylated C's in CHG context 41679
Unmethylated C's in CHH context 119949
Unmethylated C's in Unknown context 1068
Percentage methylation (CpG context) 23.2%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 7.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2880 original top strand
CTOT 252 complementary to original top strand
CTOB 248 complementary to original bottom strand
OB 2987 original bottom strand

Deduplication

Alignments analysed 6367
Unique alignments 6303
Duplicates removed 64
Duplicated alignments were found at 40 different positions

Cytosine Methylation after Extraction

Total C's analysed 147030
Methylated C's in CpG context 4930
Methylated C's in CHG context 870
Methylated C's in CHH context 7655
Unmethylated C's in CpG context 16006
Unmethylated C's in CHG context 29978
Unmethylated C's in CHH context 87591
Percentage methylation (CpG context) 23.5%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 8.0%

M-Bias Plot