Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-185_S19_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-185_S19_L003_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6151
Pairs without alignments under any condition 1970
Pairs that did not map uniquely 1879
Genomic sequence context not extractable (edges of chromosomes) 53

Cytosine Methylation

Total C's analysed 196656
Methylated C's in CpG context 5488
Methylated C's in CHG context 1109
Methylated C's in CHH context 12789
Methylated C's in Unknown context 489
Unmethylated C's in CpG context 23845
Unmethylated C's in CHG context 40687
Unmethylated C's in CHH context 112738
Unmethylated C's in Unknown context 1148
Percentage methylation (CpG context) 18.7%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 10.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2567 original top strand
CTOT 385 complementary to original top strand
CTOB 382 complementary to original bottom strand
OB 2764 original bottom strand

Deduplication

Alignments analysed 6098
Unique alignments 6025
Duplicates removed 73
Duplicated alignments were found at 34 different positions

Cytosine Methylation after Extraction

Total C's analysed 139703
Methylated C's in CpG context 4024
Methylated C's in CHG context 846
Methylated C's in CHH context 9549
Unmethylated C's in CpG context 16618
Unmethylated C's in CHG context 28366
Unmethylated C's in CHH context 80300
Percentage methylation (CpG context) 19.5%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 10.6%

M-Bias Plot