Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-184_S18_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-184_S18_L003_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6168
Pairs without alignments under any condition 1991
Pairs that did not map uniquely 1841
Genomic sequence context not extractable (edges of chromosomes) 44

Cytosine Methylation

Total C's analysed 191882
Methylated C's in CpG context 5559
Methylated C's in CHG context 996
Methylated C's in CHH context 9857
Methylated C's in Unknown context 358
Unmethylated C's in CpG context 21022
Unmethylated C's in CHG context 39860
Unmethylated C's in CHH context 114588
Unmethylated C's in Unknown context 1191
Percentage methylation (CpG context) 20.9%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 7.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2782 original top strand
CTOT 288 complementary to original top strand
CTOB 321 complementary to original bottom strand
OB 2733 original bottom strand

Deduplication

Alignments analysed 6124
Unique alignments 6052
Duplicates removed 72
Duplicated alignments were found at 36 different positions

Cytosine Methylation after Extraction

Total C's analysed 136356
Methylated C's in CpG context 4076
Methylated C's in CHG context 750
Methylated C's in CHH context 7495
Unmethylated C's in CpG context 14809
Unmethylated C's in CHG context 27762
Unmethylated C's in CHH context 81464
Percentage methylation (CpG context) 21.6%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 8.4%

M-Bias Plot