Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-181_S16_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-181_S16_L003_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6090
Pairs without alignments under any condition 2168
Pairs that did not map uniquely 1742
Genomic sequence context not extractable (edges of chromosomes) 38

Cytosine Methylation

Total C's analysed 199063
Methylated C's in CpG context 6179
Methylated C's in CHG context 1019
Methylated C's in CHH context 9447
Methylated C's in Unknown context 329
Unmethylated C's in CpG context 22976
Unmethylated C's in CHG context 41115
Unmethylated C's in CHH context 118327
Unmethylated C's in Unknown context 1095
Percentage methylation (CpG context) 21.2%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 7.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2741 original top strand
CTOT 194 complementary to original top strand
CTOB 214 complementary to original bottom strand
OB 2903 original bottom strand

Deduplication

Alignments analysed 6052
Unique alignments 5974
Duplicates removed 78
Duplicated alignments were found at 46 different positions

Cytosine Methylation after Extraction

Total C's analysed 144951
Methylated C's in CpG context 4698
Methylated C's in CHG context 794
Methylated C's in CHH context 6888
Unmethylated C's in CpG context 16513
Unmethylated C's in CHG context 29561
Unmethylated C's in CHH context 86497
Percentage methylation (CpG context) 22.1%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 7.4%

M-Bias Plot