Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-176_S15_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-176_S15_L003_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5643
Pairs without alignments under any condition 2748
Pairs that did not map uniquely 1609
Genomic sequence context not extractable (edges of chromosomes) 42

Cytosine Methylation

Total C's analysed 179532
Methylated C's in CpG context 5083
Methylated C's in CHG context 774
Methylated C's in CHH context 7747
Methylated C's in Unknown context 344
Unmethylated C's in CpG context 21581
Unmethylated C's in CHG context 36110
Unmethylated C's in CHH context 108237
Unmethylated C's in Unknown context 1005
Percentage methylation (CpG context) 19.1%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 6.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2585 original top strand
CTOT 189 complementary to original top strand
CTOB 194 complementary to original bottom strand
OB 2633 original bottom strand

Deduplication

Alignments analysed 5601
Unique alignments 5542
Duplicates removed 59
Duplicated alignments were found at 31 different positions

Cytosine Methylation after Extraction

Total C's analysed 129022
Methylated C's in CpG context 3780
Methylated C's in CHG context 600
Methylated C's in CHH context 5499
Unmethylated C's in CpG context 15383
Unmethylated C's in CHG context 25536
Unmethylated C's in CHH context 78224
Percentage methylation (CpG context) 19.7%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 6.6%

M-Bias Plot