Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-175_S14_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-175_S14_L003_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6254
Pairs without alignments under any condition 2031
Pairs that did not map uniquely 1715
Genomic sequence context not extractable (edges of chromosomes) 30

Cytosine Methylation

Total C's analysed 196517
Methylated C's in CpG context 5864
Methylated C's in CHG context 1015
Methylated C's in CHH context 9465
Methylated C's in Unknown context 336
Unmethylated C's in CpG context 21197
Unmethylated C's in CHG context 40313
Unmethylated C's in CHH context 118663
Unmethylated C's in Unknown context 1227
Percentage methylation (CpG context) 21.7%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 7.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2814 original top strand
CTOT 215 complementary to original top strand
CTOB 252 complementary to original bottom strand
OB 2943 original bottom strand

Deduplication

Alignments analysed 6224
Unique alignments 6166
Duplicates removed 58
Duplicated alignments were found at 33 different positions

Cytosine Methylation after Extraction

Total C's analysed 142689
Methylated C's in CpG context 4367
Methylated C's in CHG context 800
Methylated C's in CHH context 7055
Unmethylated C's in CpG context 15265
Unmethylated C's in CHG context 28891
Unmethylated C's in CHH context 86311
Percentage methylation (CpG context) 22.2%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 7.6%

M-Bias Plot