Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-170_S13_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-170_S13_L002_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5541
Pairs without alignments under any condition 2776
Pairs that did not map uniquely 1683
Genomic sequence context not extractable (edges of chromosomes) 42

Cytosine Methylation

Total C's analysed 166177
Methylated C's in CpG context 4588
Methylated C's in CHG context 998
Methylated C's in CHH context 10248
Methylated C's in Unknown context 517
Unmethylated C's in CpG context 18970
Unmethylated C's in CHG context 33294
Unmethylated C's in CHH context 98079
Unmethylated C's in Unknown context 1167
Percentage methylation (CpG context) 19.5%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 9.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2418 original top strand
CTOT 335 complementary to original top strand
CTOB 362 complementary to original bottom strand
OB 2384 original bottom strand

Deduplication

Alignments analysed 5499
Unique alignments 5430
Duplicates removed 69
Duplicated alignments were found at 36 different positions

Cytosine Methylation after Extraction

Total C's analysed 116993
Methylated C's in CpG context 3289
Methylated C's in CHG context 754
Methylated C's in CHH context 7877
Unmethylated C's in CpG context 13175
Unmethylated C's in CHG context 22791
Unmethylated C's in CHH context 69107
Percentage methylation (CpG context) 20.0%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 10.2%

M-Bias Plot