Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-169_S12_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-169_S12_L002_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5906
Pairs without alignments under any condition 2195
Pairs that did not map uniquely 1899
Genomic sequence context not extractable (edges of chromosomes) 17

Cytosine Methylation

Total C's analysed 181382
Methylated C's in CpG context 5320
Methylated C's in CHG context 1087
Methylated C's in CHH context 11305
Methylated C's in Unknown context 468
Unmethylated C's in CpG context 19942
Unmethylated C's in CHG context 37165
Unmethylated C's in CHH context 106563
Unmethylated C's in Unknown context 1094
Percentage methylation (CpG context) 21.1%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 9.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2556 original top strand
CTOT 333 complementary to original top strand
CTOB 327 complementary to original bottom strand
OB 2673 original bottom strand

Deduplication

Alignments analysed 5889
Unique alignments 5849
Duplicates removed 40
Duplicated alignments were found at 27 different positions

Cytosine Methylation after Extraction

Total C's analysed 129427
Methylated C's in CpG context 3850
Methylated C's in CHG context 801
Methylated C's in CHH context 8483
Unmethylated C's in CpG context 14191
Unmethylated C's in CHG context 26093
Unmethylated C's in CHH context 76009
Percentage methylation (CpG context) 21.3%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 10.0%

M-Bias Plot