Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-168_S11_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-168_S11_L002_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6077
Pairs without alignments under any condition 2103
Pairs that did not map uniquely 1820
Genomic sequence context not extractable (edges of chromosomes) 29

Cytosine Methylation

Total C's analysed 194927
Methylated C's in CpG context 6185
Methylated C's in CHG context 967
Methylated C's in CHH context 9850
Methylated C's in Unknown context 309
Unmethylated C's in CpG context 22483
Unmethylated C's in CHG context 40517
Unmethylated C's in CHH context 114925
Unmethylated C's in Unknown context 1145
Percentage methylation (CpG context) 21.6%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 7.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2767 original top strand
CTOT 220 complementary to original top strand
CTOB 218 complementary to original bottom strand
OB 2843 original bottom strand

Deduplication

Alignments analysed 6048
Unique alignments 5990
Duplicates removed 58
Duplicated alignments were found at 33 different positions

Cytosine Methylation after Extraction

Total C's analysed 139918
Methylated C's in CpG context 4538
Methylated C's in CHG context 753
Methylated C's in CHH context 7048
Unmethylated C's in CpG context 16018
Unmethylated C's in CHG context 28711
Unmethylated C's in CHH context 82850
Percentage methylation (CpG context) 22.1%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 7.8%

M-Bias Plot