Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-167_S10_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-167_S10_L002_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6346
Pairs without alignments under any condition 1856
Pairs that did not map uniquely 1798
Genomic sequence context not extractable (edges of chromosomes) 39

Cytosine Methylation

Total C's analysed 204370
Methylated C's in CpG context 6423
Methylated C's in CHG context 1384
Methylated C's in CHH context 9741
Methylated C's in Unknown context 280
Unmethylated C's in CpG context 21477
Unmethylated C's in CHG context 42067
Unmethylated C's in CHH context 123278
Unmethylated C's in Unknown context 1147
Percentage methylation (CpG context) 23.0%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 7.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2950 original top strand
CTOT 206 complementary to original top strand
CTOB 201 complementary to original bottom strand
OB 2950 original bottom strand

Deduplication

Alignments analysed 6307
Unique alignments 6273
Duplicates removed 34
Duplicated alignments were found at 25 different positions

Cytosine Methylation after Extraction

Total C's analysed 148341
Methylated C's in CpG context 4698
Methylated C's in CHG context 1027
Methylated C's in CHH context 7123
Unmethylated C's in CpG context 15600
Unmethylated C's in CHG context 30102
Unmethylated C's in CHH context 89791
Percentage methylation (CpG context) 23.1%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 7.3%

M-Bias Plot