Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-162_S9_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-162_S9_L002_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5473
Pairs without alignments under any condition 2823
Pairs that did not map uniquely 1704
Genomic sequence context not extractable (edges of chromosomes) 27

Cytosine Methylation

Total C's analysed 163512
Methylated C's in CpG context 4770
Methylated C's in CHG context 1006
Methylated C's in CHH context 11266
Methylated C's in Unknown context 452
Unmethylated C's in CpG context 17324
Unmethylated C's in CHG context 32536
Unmethylated C's in CHH context 96610
Unmethylated C's in Unknown context 1098
Percentage methylation (CpG context) 21.6%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 10.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2296 original top strand
CTOT 339 complementary to original top strand
CTOB 379 complementary to original bottom strand
OB 2432 original bottom strand

Deduplication

Alignments analysed 5446
Unique alignments 5391
Duplicates removed 55
Duplicated alignments were found at 24 different positions

Cytosine Methylation after Extraction

Total C's analysed 115647
Methylated C's in CpG context 3443
Methylated C's in CHG context 749
Methylated C's in CHH context 8463
Unmethylated C's in CpG context 12030
Unmethylated C's in CHG context 22526
Unmethylated C's in CHH context 68436
Percentage methylation (CpG context) 22.3%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 11.0%

M-Bias Plot