Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-161_S8_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-161_S8_L002_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5289
Pairs without alignments under any condition 3106
Pairs that did not map uniquely 1605
Genomic sequence context not extractable (edges of chromosomes) 25

Cytosine Methylation

Total C's analysed 160308
Methylated C's in CpG context 4635
Methylated C's in CHG context 925
Methylated C's in CHH context 10169
Methylated C's in Unknown context 506
Unmethylated C's in CpG context 17585
Unmethylated C's in CHG context 31714
Unmethylated C's in CHH context 95280
Unmethylated C's in Unknown context 1043
Percentage methylation (CpG context) 20.9%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 9.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2288 original top strand
CTOT 306 complementary to original top strand
CTOB 343 complementary to original bottom strand
OB 2327 original bottom strand

Deduplication

Alignments analysed 5264
Unique alignments 5222
Duplicates removed 42
Duplicated alignments were found at 20 different positions

Cytosine Methylation after Extraction

Total C's analysed 112022
Methylated C's in CpG context 3325
Methylated C's in CHG context 696
Methylated C's in CHH context 7698
Unmethylated C's in CpG context 12163
Unmethylated C's in CHG context 21730
Unmethylated C's in CHH context 66410
Percentage methylation (CpG context) 21.5%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 10.4%

M-Bias Plot