Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-160_S7_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-160_S7_L002_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5657
Pairs without alignments under any condition 2476
Pairs that did not map uniquely 1867
Genomic sequence context not extractable (edges of chromosomes) 42

Cytosine Methylation

Total C's analysed 173653
Methylated C's in CpG context 5108
Methylated C's in CHG context 958
Methylated C's in CHH context 10495
Methylated C's in Unknown context 431
Unmethylated C's in CpG context 19596
Unmethylated C's in CHG context 34508
Unmethylated C's in CHH context 102988
Unmethylated C's in Unknown context 1139
Percentage methylation (CpG context) 20.7%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 9.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2329 original top strand
CTOT 335 complementary to original top strand
CTOB 317 complementary to original bottom strand
OB 2634 original bottom strand

Deduplication

Alignments analysed 5615
Unique alignments 5535
Duplicates removed 80
Duplicated alignments were found at 38 different positions

Cytosine Methylation after Extraction

Total C's analysed 121757
Methylated C's in CpG context 3683
Methylated C's in CHG context 711
Methylated C's in CHH context 7920
Unmethylated C's in CpG context 13426
Unmethylated C's in CHG context 23765
Unmethylated C's in CHH context 72252
Percentage methylation (CpG context) 21.5%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 9.9%

M-Bias Plot