Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-154_S5_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-154_S5_L002_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5675
Pairs without alignments under any condition 2748
Pairs that did not map uniquely 1577
Genomic sequence context not extractable (edges of chromosomes) 40

Cytosine Methylation

Total C's analysed 177911
Methylated C's in CpG context 5775
Methylated C's in CHG context 909
Methylated C's in CHH context 8985
Methylated C's in Unknown context 418
Unmethylated C's in CpG context 18309
Unmethylated C's in CHG context 35774
Unmethylated C's in CHH context 108159
Unmethylated C's in Unknown context 1008
Percentage methylation (CpG context) 24.0%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 7.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2575 original top strand
CTOT 229 complementary to original top strand
CTOB 246 complementary to original bottom strand
OB 2585 original bottom strand

Deduplication

Alignments analysed 5635
Unique alignments 5602
Duplicates removed 33
Duplicated alignments were found at 26 different positions

Cytosine Methylation after Extraction

Total C's analysed 126843
Methylated C's in CpG context 4151
Methylated C's in CHG context 679
Methylated C's in CHH context 6544
Unmethylated C's in CpG context 12942
Unmethylated C's in CHG context 25201
Unmethylated C's in CHH context 77326
Percentage methylation (CpG context) 24.3%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 7.8%

M-Bias Plot